- Prevalence and distribution of animal and plant species in streams and ponds.
- Ecological condition of streams and ponds, assessed for the first time based on the diversity, abundance and composition of the flora and fauna*
- Physical condition of streams and their riparian corridors (ie. the land adjacent to watercourses).
- Estimates of the number of ponds.
- Links between the characteristics of catchments and the ecological status of streams within rural landscapes*
*key areas that need to be addressed as part of our drive to implement the EU Water Framework Directive
We have collected data on 455 streams & watercourses across GB

Streams
Squares that were studied during the 2000 Survey were re-surveyed in 2007 – with particular focus on sites that have a continuous time series since 1990.
Fieldwork in each square has involved:
- Sampling the stream macroinvertebrate community (eg. insects, crustaceans, molluscs and worms), using the River Invertebrate Prediction and Classification System (RIVPACS) method.
- Recording associated RIVPACS information*
- Recording the hydromorphological status of the site, using the 2003 River Habitat Survey method.
- Surveying the aquatic plant community, using the Mean Trophic Rank (MTR) method.
- Taking an indicative sample of water chemistry - analysed for pH, conductivity, alkalinity, Soluble Reactive Phosphorous (SRP) and Total Oxidised Nitrogen (TON).
*includes stream width and depth; velocity and discharge; substrate composition; altitude; distance from source; slope.

Ponds
The 2007 Survey assessed the biological condition of one pond in every square where they were found (259 in total). The study involved a new approach to biological surveying, which allows accurate assessment of change in the status of lowland ponds since 1996 – and for the first time, a UK and country-level assessment of the condition of the national pond stock.
Fieldwork at each pond has involved:
- Surveying the aquatic plant community, using the National Pond Monitoring Network (NPMN) method
- Recording associated NPMN information*
- Taking an indicative sample of water chemistry - analysed for pH, conductivity, alkalinity, Soluble Reactive Phosphorous (SRP) and Total Oxidised Nitrogen (TON).
*includes pond area; wetted area; depth; substrate composition; number of inflows/outflows; evidence of management, impacts of livestock; fish; water birds; surrounding land-use; amenity value.
Management of the pond biological survey was undertaken by Pond Conservation - the national experts on research, policy-advice and fieldwork on ponds. For more information visit the Freshwater Habitats Trust website.